In recent years, the use of plants as bioreactors has emerged as an been developed to boost up protein expression in transgenic plants. Transgenic animals as production systems: Public and ethical concernsTypes of Plant bioreactorsSeed-based plant. how plant act as bioreactor and produce r protein- authorSTREAM Presentation.


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TRANSGENIC PLANTS AS BIOREACTORS PDF DOWNLOAD


Plants as bioreactors for the production of vaccine antigens.

The transgenic plants are capable of synthesizing of many substances, including valuable pharmaceuticals. Introduction There was a time not too transgenic plants as bioreactors ago when most medicinal compounds came transgenic plants as bioreactors plants: But beginning about 50 years ago, chemistry took over from botany, with most new drugs being artificially produced in pharmaceutical labs Moffat, Nowadays, one of the most promising methods of producing proteins and other medicinal substances, such as antibodies and vaccines, is the use of transgenic plants.

A transgenic plant contains a gene or genes that has been artificially inserted. The inserted gene sequence, known as the transgene, may come from an unrelated plant, or from a completely different species. One of the purposes of inserting a combination of transgenic plants as bioreactors in a plant is to make it as useful and productive as possible.

NOPR: Transgenic plants as bioreactors

This process provides advantages such as higher yield, improved quality, pest or disease resistance, and tolerance to heat, cold and drought. However, transgenic plants can also be produced transgenic plants as bioreactors such a way that they express foreign proteins with industrial or pharmaceutical value see Tables 1 and 2.

Transgenic plants represent an economical alternative to fermentation-based production systems. Plant-made vaccines or antibodies plantibodies are especially striking, as plants are free of human diseases, thus reducing screening costs for viruses and bacterial toxins Herbers et al.

TRANSGENIC PLANTS AS BIOREACTORS PDF DOWNLOAD

The first transgenic plants were reported in Since then, many recombinant proteins have been expressed in several important agronomic species of plants including tobacco, corn, tomato, potato, banana, alfalfa and canola Hammond et al.

Transgenic plants as bioreactors choice of plant system was initially driven by convenience and the need to evaluate genetic constructs quickly.

For transgenic plants as bioreactors reason, tobacco plants were generally used, however, potatoes and bananas were considered for the purpose of acquiring a delivery system of vaccines for humans.

Foreign gene expression in plants can be accomplished transgenic plants as bioreactors by stable integration of foreign DNA, which results in transformation of the nuclear genome, or by transient expression using modified plant viruses Figure 1.

The integration method has the advantage of permitting large scale cloning, maintenance of selected high-expressing genes and the ability to sexually cross transgenes to obtain multiple proteins expressed in the same plant Tacket et al.

Transgenic Plant as Bioreactor for Recombinant Protein |authorSTREAM

On the other hand, transient transgenic plants as bioreactors using viral vectors is harder to initiate, because the viral vector must be inoculated into transgenic plants as bioreactors host plants, but gives a greater yield of protein. Advantages and Disadvantages Recombinant protein production using transgenic plants as bioreactors is likely to be more economical than alternative systems, especially for large-scale needs.

Factors in favor of plant systems as sources of animal derived proteins, compared with other conventional methods, include: The potential for large-scale, low-cost biomass production using agriculture.

Low risk of product contamination by mammalian viruses, blood-borne pathogens, oncogenes and bacterial toxins. The capacity of plant cells to correctly fold and assemble, not only antibody fragments and single chain peptides, but also full-length multimeric proteins.

Plants as bioreactors for the production of vaccine antigens.

Low downstream processing requirements for proteins administered orally. Elimination of the purification requirement when the plant containing the recombinant proteins is edible, such as potatoes. The ability to introduce new or multiple transgenes by sexual crossing of plants. transgenic plants as bioreactors

TRANSGENIC PLANTS AS BIOREACTORS PDF DOWNLOAD

The avoidance of ethical problems associated with transgenic animals. Formulated in seeds, plant-made enzymes have been found to be an extremely convenient method for reducing storage and shipping costs, for an indefinite amount of time, under ambient conditions.

Production size is flexible and easily adjustable to the transgenic plants as bioreactors of changing markets. Plants are also capable of synthesizing and assembling virtually any kind of antibody molecule, ranging transgenic plants as bioreactors the smallest antigen-binding domains and fragments, to full length, and even multimeric antibodies.



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