The pylorus ligated rat (Shay rat) was described by Shay et al. (1) for the production of acute ulcers in the forestomach, and it has been also employed as a. The antiulcer activity was carried out employing - Pyloric ligation method. Convolvulus pluricaulis, Gastric ulcers, Pyloric Ligation Method. In pylorus ligation ulcer model, various parameters were studied viz. gastric volume, pH, total acidity, free acidity, ulcer index and percentage inhibition of.


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Pyloric portion of the stomach was slightly lifted out and ligated according to method of Shay et al.

Antiulcer | Indomethacin | Oxalis Corniculata | Pylorus Ligation | Ranitidine

The stomach was replaced carefully and the abdominal wall was closed by interrupted sutures. Pyloric ligation were sacrificed by an pyloric ligation dose of anaesthetic ether after four hours of pyloric ligation. The abdomen was opened, cardiac end of the stomach was dissected out and the contents were drained into a glass tube.

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The volume of the gastric juice was measured and centrifuged Superspin, Plasto-crafts, India at rpm for 10 min. From the supernatant, aliquots 1 ml of each were taken for the determination pyloric ligation pH, total and free acidity.

Gastric acid response to pylorus ligation in rats: is gastrin or histamine involved?

The inner surface of free stomach was examined for gastric lesions. Determination pyloric ligation pH An aliquot of 1 ml gastric juice was diluted with 1 ml of distilled water and pH of the solution was measured using pH meter [ 19 ].

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Determination of total acidity An aliquot of 1 ml gastric juice diluted with 1 ml pyloric ligation distilled water was taken into a 50 ml conical flask and two drops of phenolphthalein indicator was added to it and titrated with 0.

Superoxide dismutase SOD activity was determined pyloric ligation the inhibition of autocatalysed adrenochrome formation in the presence of tissue homogenate at nm.

Glutathione reductase GSSH activity was estimated spectrophotometrically by measuring the rate of reduced nicotinamide adenine dinucleotide phosphate NADPH oxidation at nm. Glutathione peroxidase GPx activity was pyloric ligation spectrophotometrically using the following method.

Various controls were carried out and suitably subtracted.

Gastric acid response to pylorus ligation in rats: is gastrin or histamine involved?

The estimation of MDA was carried out using the TBA reactive substance test while estimation of HP was carried out after tissue homogenate was treated with Fox reagent.

Myeloperoxidase MPx activity was determined by the following pyloric ligation.

The results were considered statistically significant if the P values were less than 0. Results and Discussion Nonsteroidal analgesic and antiinflammatory drugs like aspirin can effects on oxidant and antioxidant mechanisms and interfere prostaglandin synthesis through cyclooxygenase pyloric ligation, produce neutrophil and oxygen radical dependent microvascular injury leading to mucosal damage.

In pylorus-ligated rats the gastric acid pyloric ligation is an important factor for generation of ulceration.

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When aspirin was administered to pylorus-ligated rats it further aggravated the acidity and the resistance pyloric ligation the gastric mucosa was decreased thereby pyloric ligation extensive damage to the glandular region of the stomach [ 27 ].

Aspirin plus pylorus ligation-induced animals models was used to investigate the pyloric ligation activity of gallic acid, and ulcer control group show significant differences in ulcer parameters when compared to healthy control group.

Antiulcer | Indomethacin | Oxalis Corniculata | Pylorus Ligation | Ranitidine

The glandular pyloric ligation of the stomach was then exposed and examined for macroscopic examination and ulcer index deterimination. Total acid output was estimated and gastric mucosal homogenates were prepared in normal saline.

Assessment of gross mucosal damage The gastric mucosal layer was pyloric ligation inspected for the occurrence of ulcers and their numbers were counted with the aid of an illuminated magnifying lens 10X [ pyloric ligation22 ].

The sum of the total length of long ulcers and hemorrhagic spots in each group of rats were divided by the number of animals to calculate the ulcer index mm.

Article Information

Ulcer index was calculated according to the method described by [ 23 ]. The preventive index was calculated according to the method described pyloric ligation [ 24 ].

  • Gastric acid response to pylorus ligation in rats: is gastrin or histamine involved?
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  • Gastric acid response to pylorus ligation in rats: is gastrin or histamine involved?

Gastric volume, titratable acidity, and acid output determination The collected gastric juice was centrifuged at g for 10 min and gastric volume ml was recorded after removal of solid pyloric ligation.

Samples having solid mass volumes more than 0. Titratable acidity was carried out according to the method of [ 25 ] and pyloric ligation 26 ] by titrating gastric juice against sodium hydroxide 0.

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Acid output was calculated as the rate of the gastric juice production [ 27 ] as microequivalents per 4 hours. Peptic activity determination Briefly, pepsin activity, the active principle proteolytic activity pyloric ligation gastric secretion was pyloric ligation in terms of the amount of proteases produced after incubation of the substrate with pepsin for half an hour.

The proteolytic activity of pepsin in gastric juice was determined spectrophotometrically at nm [ 28 ].



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